Structural studies on the lens β, γ crystallins
نویسندگان
چکیده
منابع مشابه
Physiological expression of lens α-, β-, and γ-crystallins in murine and human corneas
PURPOSE How corneal transparency is formed/maintained remains largely unclear. A group of enzymes which are referred to as enzymatic crystallins were proposed to contribute to corneal transparency in various animals. This study investigated whether the three classical lens crystallins, namely α-, β-, and γ-crystallins, exist in mouse and human corneas. METHODS Mice, human tissues, and culture...
متن کاملAge-dependent association of γ-crystallins with aged α-crystallins from old bovine lens
PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...
متن کاملCleavage of β-Crystallins During Maturation of Bovine Lens
Results: The identities of the major crystallins and several additional crystallin species missing portions of their Nterminal extensions were identified in the fetal bovine lens. Besides the previously identified form of βB1 missing 15 residues from its N-terminus, forms of βA3 missing 11 and 22 residues were identified. With aging, the βA3 (-22) species became a major protein in the adult bov...
متن کاملMolecular diversity and genomic organisation of the α, β and γ eye lens crystallins from the Antarctic toothfish Dissostichus mawsoni
The eye lens of the Antarctic toothfish living in the −2 °C Southern Ocean is cold-stable. To investigate the molecular basis of this cold stability, we isolated, cloned and sequenced 22 full length crystallin cDNAs. We found two α crystallins (αA, αB), six β crystallins (βA1, βA2, βA4, βB1, βB2, βB3) and 14 γ crystallins (γN, γS1, γS2, γM1, γM3, γM4, γM5, γM7, γM8a, γM8b, γM8c, γM8d, γM8e, and...
متن کاملStudies on lens proteins. I. Subunit structure of beta crystallins of rabbit lens cortex.
A method has been developed to isolate and characterize beta-crystallins of rabbit lens cortex. Chromatographic separation of water-soluble structure proteins of rabbit lens cortex on a Sephacryl S-200 gel column yielded four beta-crystallin peaks (beta1, beta2, beta3 and beta4), all eluting between alpha and gamma-crystallins. Their molecular weights were estimated to be 250,000, 130,000, 60,0...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1987
ISSN: 0108-7673
DOI: 10.1107/s0108767387084514